Chimeric (Mouse/Human) Anti-SDC1 Recombinant Antibody (TAB-197)
CAT#: TAB-197
This product is a Chimeric (mouse/human) IgG4, κ antibody that can recognize human SDC1.
Tested Data
Gene Expression
WB
Figure 1 Anti-SDC1 Antibody (TAB-197) in WB
Western blot analysis of TAB-197 was performed with Syndecan-1/CD138 Protein, Human, Recombinant (His Tag).
TAB-197 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 2 Anti-SDC1 Antibody (TAB-197) in ELISA
ELISA analysis of TAB-197 was performed by coating with Syndecan-1/CD138 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, hTCEpi, U-251 MG, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115884-SDC1
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Specifications
- Immunogen
- U266 cell line
- Host Species
- Human
- Derivation
- Chimeric (mouse/Human)
- Type
- Chimeric (mouse/Human) IgG4, κ
- Specificity
- Human SDC1
- Species Reactivity
- Human
- Applications
- Neut, ELISA, IF, IP, FuncS, FC, ICC
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Neutralization, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation, Functional Assay, Flow Cytometry, Immunocytochemistry.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-197, RRID: AB_3111866)
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Biosimilar Overview
Please refer to Indatuximab Ravtansine Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Indatuximab Ravtansine.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| TAB-495CT | Mouse Anti-SDC1 Recombinant Antibody (TAB-495CT) | FC, WB | Mouse IgG1, κ |
| TAB-495CT-S(P) | Mouse Anti-SDC1 Recombinant Antibody; scFv Fragment (TAB-495CT-S(P)) | FC, WB | Mouse scFv |
| TAB-495CT-F(E) | Mouse Anti-SDC1 Recombinant Antibody; Fab Fragment (TAB-495CT-F(E)) | FC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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| MOR-3189 | Hi-Affi™ Recombinant Rabbit Anti-SDC1 Monoclonal Antibody (DS3189AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0324-CN | Human Anti-SDC1 Recombinant Antibody (HPAB-0324-CN) | ELISA, FC | Humanized IgG3, κ |
| HPAB-0326-CN | Human Anti-SDC1 Recombinant Antibody (HPAB-0326-CN) | ELISA, FC | Humanized IgG4, κ |
| MRO-1463-CN | Recombinant Rabbit Anti-SDC1 Monoclonal Antibody (JM11-21) | WB, IF, IHC | Rabbit IgG |
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| VS7-0425-WR833 | Mouse Anti-SDC1 Recombinant Antibody (clone 1A3A3) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0324-CN-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (HPAB-0324-CN-S(P)) | ELISA, FC | Human scFv |
| HPAB-0326-CN-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (HPAB-0326-CN-S(P)) | ELISA, FC | Human scFv |
| HPAB-2546LY-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (HPAB-2546LY-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0324-CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (HPAB-0324-CN-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0326-CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (HPAB-0326-CN-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-2546LY-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (HPAB-2546LY-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-086CN | Human Anti-SDC1 Recombinant Antibody (NS-086CN) | ELISA | Humanized IgG |
| NS-087CN | Human Anti-SDC1 Recombinant Antibody (NS-087CN) | ELISA | Humanized IgG |
| NS-086CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (NS-086CN-F(E)) | ELISA | Humanized Fab |
| NS-087CN-F(E) | Human Anti-SDC1 Recombinant Antibody; Fab Fragment (NS-087CN-F(E)) | ELISA | Humanized Fab |
| NS-086CN-S(P) | Human Anti-SDC1 Recombinant Antibody; scFv Fragment (NS-086CN-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-197 | Afuco™ Anti-SDC1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-197) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1001 | Mouse Anti-SDC1 Recombinant Antibody (clone 587CT7.3.6.5) | WB, ICC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY193 | CytoStream™ Mouse Anti-SDC1 Recombinant Antibody (VS-0225-XY193) | FC | Mouse IgG1, kappa |
| VS13-YC1044 | CytoStream™ Rabbit Anti-SDC1 Recombinant Antibody (VS13-YC1044) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY16 | Human Anti-SDC1 scFv-Fc Chimera (VS-0325-FY16) | ELISA, FC, Cyt | Human IgG1, scFv-Fc |
| VS-0325-FY153 | Human Anti-SDC1 scFv-Fc Chimera (VS-0325-FY153) | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC134 | Recombinant Anti-SDC1 Vesicular Antibody, EV Displayed (VS-0425-YC134) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6401 | Anti-SDC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6402 | Anti-Mouse SDC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6404 | Anti-Rat SDC1 Immunohistochemistry Kit | IHC |
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